We have always known that both good and bad bacteria live in our small and large intestine. For many years though scientists believed they just “existed”. Not knowing how specific each individual bacteria’s role was in the human body….. all that is slowly about to change as researchers have touched (and I mean literally just touched) on the community of bacteria that actively inhabit our intestines. We are all familiar of probiotic drinks on our supermarket shelves, for example Actimel and danone to name a couple….. aimed at keeping our digestive systems functioning optimally (or so the packaging suggests) rather gimmiky I hasten to add, but never the less along the right concept. Scientists are only beginning to realise how hugely important the composition of bacteria in the gut is in regards to our immune system and overall health. It may take years and years to unwrap and discover the millions of strains in there and what they all do and how they work… but it is all very exciting, especially for autoimmune disease research. I have no doubt that these are the tracks that our new charity Auroimmune Alopecia Research UK should be following and investigating.
I stumbled across this fantastic piece of reading in the New York Times last month.
Author: Carl Zimmer
Published June 18th 2012
New York Times
For a century, doctors have waged war against bacteria, using antibiotics as their weapons. But that relationship is changing as scientists become more familiar with the 100 trillion microbes that call us home — collectively known as the microbiome.
“I would like to lose the language of warfare,” said Julie Segre,a senior investigator at the National Human Genome Research Institute. “It does a disservice to all the bacteria that have co-evolved with us and are maintaining the health of our bodies.”
This new approach to health is known as medical ecology. Rather than conducting indiscriminate slaughter, Dr. Segre and like-minded scientists want to be microbial wildlife managers.
No one wants to abandon antibiotics outright. But by nurturing the invisible ecosystem in and on our bodies, doctors may be able to find other ways to fight infectious diseases, and with less harmful side effects. Tending the microbiome may also help in the treatment of disorders that may not seem to have anything to do with bacteria, including obesity and diabetes.
“I cannot wait for this to become a big area of science,” said Michael a Fischbach, a microbiologist at the University of California, San Francisco, and an author of A Medical Ecology Manifesto published this month in the journal Science Translational Medicine.
Judging from a flood of recent findings about our inner ecosystem, that appears to be happening. Last week, Dr. Segre and about 200 other scientists published the most ambitious survey of the human microbiome yet. Known as the Human Microbiome Project it is based on examinations of 242 healthy people tracked over two years. The scientists sequenced the genetic material of bacteria recovered from 15 or more sites on their subjects’ bodies, recovering more than five million genes.
The project and other studies like it are revealing some of the ways in which our invisible residents shape our lives, from birth to death.
A number of recent reports shed light on how mothers promote the health of their children by shaping their microbiomes. In a study published last week in the journal PLoS One, Dr. Kjersti Aagaard-Tillery, an obstetrician at Baylor College of Medicine, and her colleagues described the vaginal microbiome in pregnant women. Before she started the study, Dr. Aagaard-Tillery expected this microbiome to be no different from that of women who weren’t pregnant.
“In fact, what we found is the exact opposite,” she said.
Early in the first trimester of pregnancy, she found, the diversity of vaginal bacteria changes significantly. Abundant species become rare, and vice versa.
One of the dominant species in the vagina of a pregnant woman, it turns out, is Lactobacillus johnsonii. It is usually found in the gut, where it produces enzymes that digest milk. It’s an odd species to find proliferating in the vagina, to say the least. Dr. Aagaard-Tillery speculates that changing conditions in the vagina encourage the bacteria to grow. During delivery, a baby will be coated by Lactobacillus johnsonii and ingest some of it. Dr. Aagaard-Tillery suggests that this inoculation prepares the infant to digest breast milk.
The baby’s microbiome continues to grow during breast-feeding. In a study of 16 lactating women published last year, Katherine M. Hunt of the University of Idaho and her colleagues reported that the women’s milk had up to 600 species of bacteria, as well as sugars called oligosaccharides that babies cannot digest. The sugars serve to nourish certain beneficial gut bacteria in the infants, the scientists said. The more the good bacteria thrive, the harder it is for harmful species to gain a foothold.
As the child grows and the microbiome becomes more ecologically complex, it also tutors the immune system. Ecological disruptions can halt this education. In March, Dr. Richard S. Blumberg of Harvard and his colleagues reported an experiment that demonstrates how important this education is.
The scientists reared mice that lacked any microbiome. In their guts and lungs, the germ-free mice developed abnormally high levels of immune cells called invariant natural killer T cells. Normally, these cells trigger a swift response from the immune system against viruses and other pathogens. In Dr. Blumberg’s microbe-free mice, however, they caused harmful inflammation. As adults, the mice were more likely to suffer from asthma and inflammatory bowel disease.
This experiment parallels studies of children in recent years. Children who take high levels of antibiotics may be at greater risk of developing allergies and asthma later on, many researchers have suggested.
Dr. Blumberg and his colleagues found that they could prevent the mice from becoming ill by giving them bacteria while they were still young. Acquiring a microbiome as an adult did not help the rodents.
The Good With the Bad
The diversity of species that make up the microbiome is hard to fathom. But it is even more difficult to understand how the immune system copes with this onslaught. In any one person’s mouth, for example, the scientists of the Human Microbiome Project found about 75 to 100 species. Some that predominate in one person’s mouth may be rare in another person’s. Still, the rate at which they are being discovered indicates that there may be as many as 5,000 species of bacteria that live in the human mouth.
“The closer you look, the more you find,” said Susan M. Huse of the Marine Biological Laboratory in Woods Hole, Mass., a contributor to the microbiome project.
Although the project has focused largely on bacteria, the microbiome’s diversity is wider. For example, our bodies also host viruses.
Many species in the human “virome” specialize in infecting our resident bacteria. But in the DNA samples stored in the Human Microbiome Project’s database, Kristine Wylie of Washington University and her colleagues are finding a wealth of viruses that target human cells. It is normal, it seems, for people to have a variety of viruses busily infecting their human hosts. “It’s really pretty striking that even in these healthy people, there really is a virome,” Dr. Wylie said.
The microbiome also includes fungi. In the June 8 issue of the journal Science, David Underhill, a research scientist at Cedars-Sinai hospital in Los Angeles, and his colleagues reported on a wealth of fungal species in the guts of humans and other mammals. In mice, for example, they cataloged 100 species of fungi that are new to science, along with 100 already known. This diversity is all the more remarkable when you consider that it is tolerated by an immune system that has evolved to fight off microbes. Scientists have only a dim understanding of how the system decides which to kill and which to tolerate.
Immune cells fight fungal infections, for example, with a protein called dectin-1, which attaches only to fungi. But Dr. Underhill and his colleagues found that dectin-1 is also essential for tolerating harmless fungi. When they engineered mice that couldn’t produce dectin-1, the mice responded to harmless fungi by producing so much inflammation that their own tissues were damaged.
It’s a good thing that the immune system can rein itself in, because the microbiome carries out many services for us. In the gut, microbes synthesize vitamins and break down tough plant compounds into digestible bits.
Skin bacteria are also essential, Dr. Segre said. “One of the most important functions of the skin is to serve as a barrier,” she said. Bacteria feed on the waxy secretions of skin cells, and then produce a moisturizing film that keeps our skin supple and prevents cracks — thus keeping out invading pathogens.
Restoring Order to the System
Antibiotics kill off harmful bacteria, but broad-spectrum forms can kill off many desirable species, too. Dr. Fischbach likens antibiotics to herbicides sprayed on a garden. The herbicide kills the unwanted plants, but also kills off the tomatoes and the roses. The gardener assumes that the tomatoes and roses will grow back on their own.
In fact, there’s no guarantee the microbial ecosystem will automatically return to normal. “It’s one of those assumptions we make today that will seem silly in retrospect,” Dr. Fischbach said. Indeed, some bacteria are adapted for invading and establishing themselves in disrupted ecosystems. A species called Clostridium difficile will sometimes invade a person’s gut after a course of antibiotics. From 2000 to 2009, the number of hospitalized patients in the United States found to have C. difficile more than doubled, to 336,600 from 139,000. Once established, the antibiotic-resistant C. difficile can be hard to eradicate.
Now that scientists are gaining a picture of healthy microbiomes, they are optimistic about restoring devastated ones. “I don’t know that we’re quite on the cusp of being able to do that well at this point. But I think at least the data is starting to argue that these might be possibilities,” said Barbara Methé of the J. Craig Venter Institute, a principal investigator on the microbiome project.
One way to restore microbiomes may be to selectively foster beneficial bacteria. To ward off dangerous skin pathogens like Staphylococcus aureus, for instance, Dr. Segre envisions applying a cream infused with nutrients for harmless skin bacteria to feed on. “It’s promoting the growth of the healthy bacteria that can then overtake the staph,” she said.
Adding the bacteria directly may also help. Unfortunately, the science of so-called probiotics lags far behind their growth in sales. In 2011, people bought $28 billion of probiotic foods and supplements, according to the research firm EuroMonitor International. But few of them have been tested as rigorously as conventional drugs.
“I think the science has been shoddy and flimsy,” said Dr. Fischbach (who is on the scientific advisory board of Schiff Nutrition International).
Nonetheless, he sees a few promising probiotic treatments. A growing number of doctors are treating C. difficile with fecal transplants: Stool from a healthy donor is delivered like a suppository to an infected patient. The idea is that the good bacteria in the stool establish themselves in the gut and begin to compete with C. difficile. This year, researchers at the University of Alberta reviewed 124 fecal transplants and concluded that the procedure is safe and effective, with 83 percent of patients experiencing immediate improvement as their internal ecosystems were restored.
Dr. Alexander Khoruts of the University of Minnesota and his colleagues want to make fecal transplants standard practice. They can now extract bacteria from stool, “removing the ‘ick’ factor,” as he puts it.
Dr. Khoruts and his colleagues have federal approval to start formal clinical trials on fecal transplants. Eventually, he would like to develop probiotic pills that contain just a few key species required to build the intestinal ecosystem.
“People are starting to take this seriously,” Dr. Fischbach said. “This is a therapy that’s going to help a lot of people.”
Other conditions potentially could be treated by manipulating the microbiome. Scientists have linked obesity, for example, to changes to the gut’s ecosystem. When scientists transfer bacteria from obese mice to lean ones, the lean mice put on weight.
How this happens is still unclear, but some studies suggest that an “obese” microbiome sends signals to the body, changing how cells use sugar for energy and leading the body to store extra fat.
Researchers at the Academic Medical Center in Amsterdam are running a clinical trialto see if fecal transplants can help treat obesity. They have recruited 45 obese men; some are getting transplants from their own stool, while others get transplants from lean donors. The scientists are finding that the transplants from lean donors are changing how the obese subjects metabolize sugar.
While these initial results are promising, there is no evidence yet that the obese subjects are losing weight. Dr. Fischbach cautions that it may take a while to figure out how to manipulate the microbiome to make people healthy.
And it may take even longer to persuade doctors to think like ecologists.
“The physicians I know really like things that are clear and crisp,” Dr. Fischbach said. “But like any ecosystem, the microbiome is not the kind of place to find simple answers.”
Could an over population of a “bad” bacteria be causing our immune systems to malfunction and produce an extreme inflammatory response over our skin? Have one of the many triggers of alopecia caused a shift in the composition of bacteria in the gut resulting in this? I think it is definitely worth a look… Don’t you?